Cholinesterases from Plant Tissue
نویسندگان
چکیده
منابع مشابه
Cholinesterases from plant tissue: v. Cholinesterase is not pectin esterase.
Several properties of the cholinesterase from Phaseolus aureus Roxb. and of pectin (methyl) esterases from both Phaseolus aureus and Lycopersicon esculentum (L.) Mill. are contrasted. Cholinesterase activity is inhibited by all of the concentrations of NaCl tested, from 0.05 m to 0.9 m, a property which differs sharply from published data pertaining to pectin esterase. Although crude preparatio...
متن کاملCholinesterases from plant tissues: I. Purification and characterization of a cholinesterase from mung bean roots.
A cholinesterase was purified 36-fold from mung bean (Phaseolus aureus) roots by a combination of differential extraction media and gel filtration. The enzyme could be effectively extracted only by high salt concentration, indicating that it is probably membrane-bound. Methods used for assaying animal cholinesterases were tested, two of which were adapted for use with the bean cholinesterase. T...
متن کاملTissue cholinesterases. A comparative study of their kinetic properties.
The substrate saturation and temperature-dependent kinetic properties of soluble and membrane-bound forms of acetylcholinestarase (AChE) from brain and butyrylcholinesterase (BChE) from heart and liver were examined. In simultaneous studies these parameters were also measured for AChE in erythrocyte membranes and for BChE in the serum from rat and humans. For both soluble and membrane-bound for...
متن کاملBlood Cholinesterases from Washington State Orchard Workers
Court-ordered monitoring of blood cholinesterases (ChEs) from orchard workers in Washington State is underway. In 2008, the mean red blood cell acetylcholinesterase (AChE, EC 3.1.1.7) activity was 9.65 +/- 1.11 micromoles/min/ml (n = 1,793) and the mean serum (BChE, 3.1.1.6) activity was 5.19 +/- 0.90 micromoles/min/ml (n = 1,811). Determinations were made using the Ellman assay and automated e...
متن کاملThe cholinesterases.
In 1914 Sir Henry Dale (1) suggested that an enzyme which degrades the esters of choline played a role in neurotransmission within the autonomic and somatic motor nervous systems and that this enzyme, acetylcholinesterase, was the target of action of the drug, physostigmine (eserine). In the intervening 75 years, inhibition of the enzyme has been used to augment both the nicotinic and muscarini...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1974
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.54.5.797